Latest News & Events

Royal Summer Society Summer Exhibition Video

24 June 2015

Check out our new video for the RSSE!

Single Cell Science at the Royal Society Summer Exhibition, 29 June – 5 July 2015 .

News: Visit us at the Royal Summer Science Exhibition 2015!

24 June 2015

We have been selected to present at this years Royal Summer Society Exhibition.
The exhibition is free and open to the public from the 30th of June to the 5th July.

News: Professor David Klug to present at the 2nd Annual Single Cell Genomics & Transcriptomics Asia Congress 2014, Singapore

1 September 2014

Professor David Klug is invited to present at 2nd Annual Single Cell Genomics & Transcriptomics Asia Congress 2014, Singapore on 7-8 October 2014.

“Progress Towards Point of Care Methods for Single Cell Protein Analysis”

  • Analysis of rare cells or heterogenous cell responses can have clinical value
  • Analysis of rare cells or heterogeneous cell responses also has value in terms of understanding basic biology
  • In the case of the analysis of circulating tumour cells it would be ideal to be able to provide information to clinicians during the visit of the patient to the clinic
  • We report progress on microfluidic technology incorporating single molecule detection which we hope will ultimately be of use in both the clinical and the basic research contexts

News: Dr Corinne Spickett will be chairing at SFRR-Europe annual conference

29 August 2014

Dr Corinne Spickett will be organising and chairing a symposium on Protein Lipoxidation: biological role and analysis at the SFRR-Europe annual conference in Paris. https://www.sfrr-europe.org/congresses/

 

News: Proxo member invited to organize workshop on Translation of Redox Methodology from Bench to Bedside – Problems and Solutions

29 August 2014

Dr Corinne Spickett has been invited to organize the pre-meeting workshop on Translation of Redox Methodology from Bench to Bedside – Problems and Solutions at the SFRBM conference in Seattle http://www.sfrbm.org/sections/annual-meeting/21st-annual-meeting and invited to talk on Advantages and Limitations of Mass Spectrometry as a Diagnostic Tool for Redox Biology

 

News: Proxo publication

21 August 2014

Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment

Mairi E. Sandison, Karina Tveen Jensen, Frank Gesellchen, Jonathan M. Cooper and Andrew R. Pitt. http://pubs.rsc.org/en/content/articlelanding/2014/an/c4an00750f#divAbstract

Reversible phosphorylation plays a key role in numerous biological processes. Mass spectrometry-based approaches are commonly used to analyze protein phosphorylation, but such analysis is challenging, largely due to the low phosphorylation stoichiometry. Hence, a number of phosphopeptide enrichment strategies have been developed, including metal oxide affinity chromatography (MOAC). Here, we describe a new material for performing MOAC that employs a magnetite-doped polydimethylsiloxane (PDMS), that is suitable for the creation of microwell array and microfluidic systems to enable low volume, high throughput analysis. Incubation time and sample loading were explored and optimized and demonstrate that the embedded magnetite is able to enrich phosphopeptides. This substrate-based approach is rapid, straightforward and suitable for simultaneously performing multiple, low volume enrichments.

 

News: Proxomics team at the 72nd World Science Fiction Convention, ExCeL, London Dockland

16 August 2014

The whole Proxomics team exhibited at the Flexible Space at 72nd World Science Fiction Convention at ExCeL, London Dockland, from 14-16 August 2014.

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There were practical demonstrations including an optical trapping deck (a tiny tractor beam), a model mass spectrometer and a model to demonstrate Surface Acoustic Waves (SAW).

 

&#169Video made by TAJAYI Media (www.tajayi.com) Music:”Ouroboros” Kevin MacLeod (incompetech.com) Licensed under Creative Commons: By Attribution 3.0 http://creativecommons.org/licenses/by/3.0/

 

http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/eventssummary/event_11-8-2014-16-52-16

News: Job opportunity at Imperial

11 August 2014

Current vacancy:
http://www.jobs.ac.uk/job/AJH448/proxomics-project-manager/

Proxomics Project Manager
(Fixed term appointment from 1 September 2014 until 29 February 2016)
Imperial College London

For more information and details on how to apply, please visit www.imperial.ac.uk/employment, click ‘job search’ and enter NS 2014 155 AS in ‘keywords’. The closing date is 17 August 2014 (Midnight BST).

Interviews will be held during the week beginning 25 August 2014.

News: Proxo publication

7 August 2014

Addressable Droplet Microarrays for Single Cell Protein Analysis

Ali Salehi-Reyhani, Edward Burgin, Oscar Ces, Keith R Willison and David Klug. Analyst, 2014, DOI: 10.1039/C4AN01208A. http://pubs.rsc.org/en/content/articlelanding/2014/an/c4an01208a#!divAbstract

Addressable droplet microarrays are potentially attractive as a way to achieve miniaturised, reduced volume, high sensitivity analyses without the need to fabricate microfluidic devices or small volume chambers. We report a practical method for producing oil-encapsulated addressable droplet microarrays which can be used for such analyses. To demonstrate their utility, we undertake a series of single cell analyses, to determine the variation in copy number of p53 proteins in cells of a human cancer cell line.

News: Proxomics team at Glasgow public engagement event

6 July 2014

Rab Wilson and Frank Gesellchen demonstrated the SAW technology at a local public engagement event in Glasgow Science Centre on 5-6 July.

News: Chair of External Advisory Board gave careers talk to Proxomics Fellows

19 June 2014

The Chair of External Advisory Board, Dr Malcolm Weir from Heptares Therapeutics, spent the day with Proxomics Fellows on 19 June 2014.  He gave an inspiring careers talk and met the Fellows individually.

News: Professor Keith Willison to present at the Quantitative Digital Detection Technologies Conference, Boston

2 June 2014

Professor Keith Willison is invited to present at Cambridge Healthtech Institute’s Second Annual Quantitative Digital Detection Technologies conference, Boston, on 17-18 June 2014

“Quantitative Single Cell and Single Molecule Proteomics for Clinical Studies”

We have developed a label free, microfluidic antibody capture chip platform called the MAC chip to quantify precisely the copy numbers of several proteins from a single cell in a multiplexed single assay format. We are extending the capability of the MAC chip devices in order to count proteins in circulating tumour cells (CTCs) isolated from biopsies of cancer patients undergoing chemotherapy. We hope to discover biomolecular signatures which correlate with intrinsic biological properties of tumour cells and clinical outcomes during treatment.

News: Professor David Klug to present at the 2014 ITMAT, Penn

2 June 2014

Professor David Klug is invited to present at the 9th Annual International Symposium of the Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, USA on 13-14 October 2014.

News: Professor David Klug to present at the Royal Society of Chemistry Analytical Research Forum, London

30 May 2014

Professor David Klug is invited to present at Royal Society of Chemistry Analytical Research Forum, London, on 7 July 2014

“Microfluidics and Miniaturised Assays for Single Cell Analysis: Applications to Basic Biology and Clinical Challenges”

We have been exploring the utility of miniaturising biochemical capture assays using a combination of microfluidics and single molecule detection. One application of this approach is to study cell to cell variation in phenotype.   In this talk I present progress with the technology and show how it can be used to analyse cell to cell variations in cell lines and the biological interpretation of these results. I also present some preliminary data from clinical samples where we are using our chip technology to study tumour cell heterogeneity. The aim in this case is to better understand the cancer biology at work and provide a practical window into disease progression in order to assist clinicians with disease management.

News: Anywhere HPLC wins top prize at Big Idea Bootcamp

28 May 2014

AnywhereHPLC won first place at Climate-KIC UK’s Big Idea Bootcamp, winning €20 000 and entry into Stage 1 of the UK Accelerator programme.

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PROXO PDRAs Drs Duncan Casey and Ali Salehi-Reyhani from the Institute of Chemical Biology presented AnywhereHPLC’s  handheld, disposable device which connects to a smart phone, allowing researchers to analyse mixtures, such as groundwater or blood, anywhere for instant results, removing the need to transport samples back to laboratories and helping prevent the environmental effects of over-fertilisation amongst other related benefits.

http://www.climate-kic.org/news/anywherehplcs-big-idea-wins-them-a-spot-on-the-climate-kic-uk-accelerator-programme/

http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/naturalsciences/chemistry/newssummary/news_28-5-2014-14-19-18

http://www.imperialinnovations.co.uk/news-centre/news/anywherehplc-wins-top-prize-innovation-programme/

News: Accepted for poster at the 2014 International Biophysics Congress

23 May 2014

Dr Duncan Casey will be presenting at the 2014 International Biophysics Congress, Brisbane, Australia, 3-7 August 2014.

“Smart Droplet Microtools: an optically controlled platform for single-cell sampling and payload delivery”

The study of cellular heterogeneity and analysis of rare cell types has the potential to deliver great insights into a number of disease states, particularly in conditions associated with aging and cancer. However, the technical challenges associated with such small systems mean that a whole new toolkit is required to extract meaningful, quantitative data. As a result, we have developed a suite of optically-manipulated Smart Droplet Microtools (SDMs) capable of sampling from or delivering to the membrane of an individual cell amongst a population, in a manner that is non-destructive, temporally resolved and repeatable. We have demonstrated the collection of inner-leaflet proteins and their deposition onto supported bilayers for later study, and have used related technologies to deliver known quantities of plasmid to single cells for quantitative dose-response transfection studies.

This means that for the first time we are capable of studying the responses of individual cells to stimuli in a way that lays bare their heterogeneity and which, when coupled with the single-molecule-sensitivity of the group’s microfluidic antibody capture chip technology, will lead to quantitative readout of cellular function throughout any given cell’s life cycle.

News: PROXO member presented at the Single Cell Analysis Conference, Cambridge, Massachusetts, USA

23 May 2014

Dr Oscar Ces presented at the Single Cell Analysis Conference, Cambridge, Massachusetts, USA, 22-23 May 2014.

“Novel Tools and Technologies for Single Cell Proteomics Analysis”

We have developed a generic platform to undertake the analysis of protein copy number from single cells. The approach described here is ‘all-optical’ whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device.

In addition, we have developed Smart Droplet Microtools (SDMs), oil micro-droplets coated with amphiphilic monolayers that are manipulated using optical traps in microfluidic environments. These enable us to perform biopsies on the cellular scale. We have demonstrated that following controlled cell-SDM interactions protein transfer to the SDM from the target cell occurs via specific contact points with the SDMs acting as storage vehicles for the uptaken plasma membrane material.

News: : Congratulations to Proxomics PhD students for winning prizes for Best Presentation at Inter-CDT Conference, Warwick

2 May 2014

Congratulations to Proxomics students for winning prizes at the Inter-CDT Conference at Warwick University on 1-2 May 2014. Mike Barclay won a prize for the Best cheap essay writing service uk Presentation and Doug Wylie won the Runner Up Presentation prize

News: Proxomics Team Meeting held at The Burn, Glenesk, Brechin, Angus

17 April 2014

The Proxomics Team Meeting was held at The Burn, Angus on 15-17 April 2014. The meeting was attended by all members from Glasgow, Aston and Imperial. After the welcome and introduction of new member of the Group (Dr Inna Guterman at Imperial), PDRAs and students presented a work update followed by a general group discussion.

News: PROXO publication

9 April 2014

Spatially Selecting a Single Cell for Lysis Using Light-Induced Electric Fields

C. Witte, C. Kremer, M. Chanasakulniyom, J. Reboud, R. Wilson, J. M. Cooper, S. L. Neale, Small, 2014, DOI: 10.1002/smll.201400247 http://onlinelibrary.wiley.com/doi/10.1002/smll.201400247/full

An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.

News: PROXO member presented a poster at the SFRRI Meeting, Kyoto, Japan

31 March 2014

Ivan Verrastro presented a poster at the SFRRI Meeting in Kyoto, Japan, on 23-26 March 2014.

“Analysis of PTEN protein-protein interactions in its reduced and oxidized form by GSH affinity enrichment and MS-based label free protein quantitation”

PTEN (Phosphatase and tensin homolog) is a redox-sensitive dual-specificity protein phosphatase involved in a number of key cellular
processes including metabolism, apoptosis and cell proliferation. It acts as a tumor suppressor by negatively regulating the PI3K/Akt pathway.  Evidence of redox regulation of downstream signaling via the formation of a regulatory disulfide has been reported, but the effect of targeted oxidation of PTEN on its interactome is still poorly described.

We have developed an experimental system to investigate the correlation between PTEN redox status and its protein-protein interactions. We affinity-enriched a HCT116 cell lysate with immobilized PTEN in its reduced and H2O2-oxidized form. PTEN oxidation status was determined by phosphatase activity and mass spectrometry. Interacting proteins were then analyzed by LC-MS using label-free quantification.

A number of protein interactions that vary with redox status of the PTEN were identified and a limited number of these have been validated. Studies of the functional significance are ongoing.

Our results suggest that H2O2 treatment causes a functional variation in the PTEN interactome that might be important for PTEN
activity and signaling. The method could be useful for the study of protein-protein interaction under oxidative stress conditions.

News: PROXO publication

20 March 2014

The Grab-and-Drop Protocol: A Novel Strategy for Membrane Protein Isolation and Reconstitution from Single Cells

A. Schrems, J. Phillips, D. R. Casey, D. Wylie, M. Novakova, U. B. Sleytr, D. Klug, M. A. A. Neil, B. Schuster and O. Ces, Analyst, 2014, DOI: 10.1039/C4AN00059E http://pubs.rsc.org/en/content/articlelanding/2014/an/c4an00059e#!divAbstract

We present a rapid and robust technique for the sampling of membrane-associated proteins from the surface of a single, live cell and their subsequent deposition onto a solid-supported lipid bilayer. As a proof of principle, this method has been used to extract green fluorescent protein (EGFP) labelled K-ras proteins located at the inner leaflet of the plasma membrane of colon carcinoma cells
and to transfer them to an S-layer supported lipid bilayer system. The technique is non-destructive, meaning that both the cell and proteins are intact after the sampling operation, offering the potential for repeated measurements of the same cell of interest. This system provides the ideal tool for the investigation of cellular heterogeneity, as well as a platform for the investigation of rare cell types such as circulating tumour cells.

News: PROXO publication

12 March 2014

Amphiphilic drug interactions with model cellular membranes are influenced by lipid chain melting temperature

D. Casey, K.

scientific thesis

Charalambous, A. Gee, R. V. Law, O. Ces, J. R. Soc. Interface, 2014, DOI: 10.1098/​rsif.2013.1062. http://rsif.royalsocietypublishing.org/content/11/94/20131062.full

Small-molecule amphiphilic species such as many drug molecules frequently exhibit low-to-negligible aqueous solubility, and generally have no identified transport proteins assisting their distribution, yet are able to rapidly penetrate significant distances into patient tissue and even cross the blood–brain barrier. Previous work has identified a mechanism of translocation driven by acid-catalysed lipid hydrolysis of biological membranes, a process which is catalysed by the presence of cationic amphiphilic drug molecules. In this study, the interactions of raclopride, a model amphiphilic drug, were investigated with mixtures of biologically relevant lipids across a range of
compositions, revealing the influence of the chain-melting temperature of the lipids upon the rate of acyl hydrolysis.

News: PROXO publication

11 March 2014

Absolute Quantification of Protein Copy Number using a Single-Molecule-Sensitive Microarray

E. Burgin, A. Salehi-Reyhani, M. Barclay, A. T. Brown, J. Kaplinsky, M. Novakova, M. A. A. Neil, O. Ces, K. R. Willison and D. Klug, Analyst, 2014, DOI: 10.1039/C4AN00091A. http://pubs.rsc.org/en/content/articlelanding/2014/an/c4an00091a#!divAbstract

We report the use of a microfluidic microarray incorporating single molecule detection for the absolute quantification of protein copy number in solution. In this paper we demonstrate protocols which enable calibration free detection for two protein detection assays. An EGFP protein assay has a limit of detection of <30 EGFP proteins in a microfluidic analysis chamber (limited by non- specific background binding), with a measured limit of linearity of approximately 6×106 molecules of analyte in the analysis chamber and a dynamic range of >5 orders of magnitude in protein concentration. An antibody sandwich assay was used to detect unlabelled human tumour suppressor protein p53 with a limit of detection of approximately 21 p53 proteins and a dynamic range of >3 orders of magnitude. We show that these protocols can be used to determine the absolute protein copy number at the single cell level in two human cancer cell lines.

Event: The Protein-Protein Interaction 3rd Young Researchers Symposium

7 March 2014

Network’s 3rd Young Researchers Symposium will be held on the Monday 7th of April 2014 at Imperial College London.  This symposium provides an opportunity for scientists from different backgrounds to meet, share and discuss research on protein-protein interactions. Keynote talks for the day will be given by Prof. Geoff Barton from Dundee, Dr Xavier Morelli from CNRS France, and Dr Rivka Isaacson from King’s College London. 

Abstract submission for short talks (20 minutes) or poster from PhD students and post-docs are highly encouraged as this will serve an excellent platform to present your research to a wider audience from across the UK. Attendance to the symposium is free, and the closing date for abstract submission (talks and posters) is Thursday 13th March 2014.

For registration to this event, please visit the following website: http://ppi-net.org/events/

News: New Research member of staff joined PROXO at Imperial

3 March 2014

Dr Inna Guterman joined the Proxomics Project at Imperial College.

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News: PROXO member invited to present at Lab-on-a-Chip European Congress

1 March 2014

Professor Jon Cooper presented at the Lab-on-a-Chip European Congress in Berlin on 10-11 March 2014.

“Multiplexed Infectious Disease Testing – Sound Point-of-Care Diagnostics”

We have developed a new technology which enables us to shape acoustic fields and tailor their interaction with fluids. We have now demonstrated the concept on a range of medical diagnostics, from in vivo glucose sensing to molecular assays, enabling sample reparation, reagent actuation and sensing. We currently have a range of projects exploring multiplexed clinical testing for malaria, tuberculosis and sexually transmitted diseases, with NHS partners.

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http://selectbiosciences.com/conferences/agendaAbstracts.aspx?conf=loacec2014&AbstractID=5479

News: PROXO member invited to talk at Single Cell Analysis Europe Conference 2014

1 March 2014

Professor David Klug presented at the Single Cell Analysis Europe Conference in Berlin on 10-11 March 2014.

Towards Single Cell Analysis of Proteins from Clinical Samples to Human Health

Over the past few years we have been developing microfluidic devices to support the quantification of protein copy number from single cells. There are multiple motivations for the development of such technologies. Cell to cell variation and the correlation between such variations can reveal the connectivity of a signalling/regulatory pathway or network within the cell. Alternatively there are many cases where only small numbers of cells are available for study and sensitivity is therefore at a premium. There is also the case of the analysis of clinical material from patients. In these cases minimally invasive biopsies are highly desirable, as is the ability to maximise the useful information that can be obtained. In this talk I discuss progress with the technology so far and give examples of the latest methods in use in my lab for single cell protein analysis together with the cell handling techniques which are a practical requirement of undertaking such studies. I also show preliminary data from clinical samples to illustrate how single cell analysis techniques can be put to use in the field of biomedical research and healthcare.

https://selectbiosciences.com/conferences/agendaAbstracts.aspx?conf=SCAE2014&AbstractID=5651

News: PROXO member invited to present at the Conway Institute, University College Dublin

1 March 2014

Professor David Klug presented at the Conway Institute, University College Dublin in Dublin, Ireland, on 18 March 2014

“The Why, How and What of Single Cell Analysis: Towards Routine Analysis of Human Tissue Relevant to Human Health”

Nearly all human tissues are a mix of cell type. Moreover even cells of the same type taken from the same organ can show significant variation in phenotype. This complexity is particularly evident in cancer where heterogeneity in genome adds considerable complexity to the classification of cancers and in developing therapeutic interventions.

Although it is now possible to undertake single cell sequencing with relatively little difficulty, single cell protein analysis remains highly challenging. I will discuss the background to the study of cell heterogeneity in cancer and present our progress towards a microfluidic
analysis platform with the potential to analyse a range of clinical material both to understand the biology better and also to monitor patient progression and help to personalise their treatment.

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News: PROXO member invited to present at Society for Free Radical Research Europe 2013 Conference

20 August 2013

Corinne M Spickett – Protein modification and phospholipid oxidation. Society for Free Radical Research Europe 2013 Conference Athens 23-25th September 2013.

News: PROXO member invited to present at HUPO Conference

20 August 2013

David Klug – Single Cell Analysis of Proteins from Clinical Samples Relevant to Human Health, HUPO Conference, Yokohama, Japan, 14-18 September 2013. http://www.hupo2013.com

News: PROXO publication

20 August 2013

Keith Willison, David Klug. Quantitative single cell and single molecule proteomics for clinical studies. Curr. Opin. Biotech. 2013, 24 (4) 745-751 (DOI: 10.1016/j.copbio.2013.06.001).

http://www.sciencedirect.com/science/article/pii/S0958166913005843#

News: Proxomics Away Day

16 August 2013

The Proxomics away day was held on 14 & 15 August 2013 at Chicheley Hall. The meeting was attended by all PROXO members, PIs, PDRAs and students from Aston, Glasgow and Imperial. After the welcome and introduction of new members of the Group (Drs Frank Gelleschen & Dileep Mampallil, PDRA’s at Glasgow, and Dr Agostino Cilibrizzi, PDRA at Imperial), the PIs provided updates on the Proxomics milestones. This was followed by other sessions, including a “sticky stepping” exercise for creating new milestones, a journal club, and general discussion.

News: New Research staff join PROXO in Glasgow

20 July 2013

Dr Dileep Mampallil Augustine and Dr Frank Gesellchen have joined the Proxomics group at the University of Glasgow.

News: PROXO publication

20 July 2013

Karina Tveen-Jensen, Ana Reis, Laetitia Mouls, Andrew R. Pitt, Corinne M. Spickett. Reporter ion-based mass spectrometry approaches for the detection of non-enzymatic protein modifications in biological samples. J. Prot. 2013, S1874-3919(13)00180-2 (DOI: 10.1016/j.jprot.2013.03.033). http://www.sciencedirect.com/science/article/pii/S1874391913001802

News: PROXO member invited to present at Max Born Institute Colloquium

26 June 2013

David Klug – Biological Problems That Can Be Solved with 2DIR Spectroscopy: Fact or Fiction?, Max Born Institute Colloquium, Berlin, Germany, 26 June 2013. http://www.mbi-berlin.de/en/events/colloquium/

Event: PPI-Net International Conference

20 June 2013

PPI-Net International Conference

10th September 2013, University of Leeds

www.ppi-net.org

News: PROXO member invited to present at Single Cell Genomics and Transcriptomics Asia Congress

14 June 2013

David Klug – Single Cell Protein Analysis from Clinical Samples Relevant to Human Health, Single Cell Genomics and Transcriptomics Asia Congress, Singapore, 13-14 June 2013. http://www.singlecellasia-congress.com

News: Job opportunity at Imperial

20 May 2013

Current vacancy:
http://www.jobs.ac.uk/job/AGN785/institute-of-chemical-biology-project-manager/

Institute of Chemical Biology Project Manager
(Temporary appointment for 7 months, Maternity cover from 1 July 2013)
Imperial College London

For more information and details on how to apply, please visit www.imperial.ac.uk/employment, click ‘job search’ and enter NS 2013 105 AS in ‘keywords’. The closing date is 30 May 2013 (Midnight BST).

News: PROXO member invited to present at 2nd International Conference on Plant Chemical Biology

17 May 2013

David Klug – The Potential for using Two-Dimensional Infrared Spectroscopy as a bioanalytical tool, 2nd International Conference on Plant Chemical Biology, Syngenta International Research Centre, Bracknell, Jealott’s Hill Berkshire, 17 May 2013. http://www.agri-net.net/events/agri-net-international-conference-on-plant-chemical-biology-jeallots-hill-internation-research-centre

News: Job opportunity at Imperial

30 April 2013

Current vacancy:
http://www.jobs.ac.uk/job/AGK721/research-associate-in-synthetic-chemical-biology/

Research Associate in Synthetic Chemical Biology
Institute of Chemical  Biology, Imperial College London

For more information and details on how to apply, please visit www.imperial.ac.uk/employment, click ‘job search’ and enter NS 2013 089 AS in ‘keywords’. The closing date is 27 May 2013 (Midnight BST).

News: PROXO member invited to present at OSA Optics in Life Sciences Conference

18 April 2013

Mark Neil – Optical Manipulation of Chemically Functionalised Emulsions and Suspensions for Single Cell Analysis, OSA Optics in Life Sciences Conference, Hawaii, 14-18 April 2013. http://www.osa.org/en-us/meetings/optics_and_photonics_congresses/optics_in_the_life_sciences/

News: PROXO member invited to present at Joint Meeting of the British and German Biophysical Society

23 March 2013

David Klug – Electron-Vibration-Vibration coupling as readout signature for biomolecular composition, structure and dynamics, Joint Meeting of the British and German Biophysical Society, Hünfeld, Germany, 21-23 March 2013. http://www.biophysik.uni-konstanz.de/biophysics-meeting/registration-and-abstract-submission/

News: PROXO member invited to present at University of Nottingham

6 March 2013

David Klug – EVV 2DIR Spectroscopy for the detection and analysis of Biomolecular complexes, geometry/structure determination and analytical studies of proteins and metabolites, School of Chemistry, University of Nottingham 6 March 2013.

News: PROXO publication

5 March 2013

Ali Salehi-Reyhani, Sanjiv Sharma, Edward Burgin, Michael Barclay, Anthony E.G. Cass, Mark A.A. Neil, Oscar Ces, Keith R Willison and David Klug. Scaling Advantages and Constraints in Miniaturized Capture Assays for Single Cell Protein Analysis. Lab Chip 2013. DOI: 10.1039/C3LC41388H.

Single cell analysis is a demanding challenge.  Research groups involved in quantitative single cell analysis, including ourselves, are beginning to take the next step which is to multiplex in order to measure as many different proteins from a single cell at once.  Knowing how immunoassay performance scales with sensor and device geometry is very important for this next step; researchers must be acutely aware of the advantages and limitations that miniaturisation brings.  Although we make specific reference to single cell immunoassays, we have presented our manuscript in a manner which we hope will benefit readers who are interested in developing miniaturised capture-based assays of any type.

http://pubs.rsc.org/en/Content/ArticleLanding/2013/LC/C3LC41388H

News: PROXO member invited to present at Lab-on-a-Chip European Congress

5 March 2013

Jon Cooper – Using Phononic Crystals to Shape Complex Fluid Flows, Lab-on-a-Chip European Congress, Barcelona, Spain, 4-5th March 2013.

News: PhD Studentship

13 February 2013

PhD Studentship is available at the University of Glasgow.
The advert can be viewed here: http://www.findaphd.com/search/ProjectDetails.aspx?PJID=43374&LID=545

Closing deadline for applications: 28 February 2013

News: Job opportunities at Glasgow

13 February 2013

Current vacancies:

Research Assistant/Associate

College of Science & Engineering, School of Engineering, University of Glasgow

For more details, please visit the Jobs at Glasgow website

Closing date for applications: 14th March 2013

News: PROXO member invited to present at VIPCA conference on Chemical Biology: Methods and Progress

12 February 2013

Rudiger Woscholski – Pleckstrin homology domain mimicry: chemical biology of phosphoinositide signalling, VIPCA conference on Chemical Biology: Methods and Progress, Vienna University, Austria, 11-12 February 2013. http://vipca.at/conferences/past_conferences.php?year=2013&conference=1&page=1

News: PROXO member invited to present at COST CM1001 Workshop

14 January 2013

Corinne Spickett – Challenges and successes in analysing adducts between proteins and phospholipid oxidation products, COST CM1001 Workshop, The Hebrew University, Jerusalem, 14 January 2013.

News: PROXO member invited to present at COST CM1001 Workshop

14 January 2013

Karina Tveen Jensen – Mass spectrometry and proteomic approaches to identity oxidative protein modifications, COST CM1001 Workshop, The Hebrew University, Jerusalem, 14 January 2013. (Invited talk)

News: PROXO member invited to present at 4th international Symposium of the Lowe syndrome Trust

14 December 2012

Rudiger Woscholski – PH-domain mimetics: potential drugs for Lowe Syndrome?, 4th international Symposium of the Lowe syndrome Trust – Molecular and clinical advances in Lowe Syndrome, Royal Society London, UK, 14 December 2012. http://www.lowetrust.com/build/pdfs/LST%20Symposium%20invitation%202012.pdf

Event: PPI-Net International Conference

27 November 2012

“Protein-protein interactions: Emerging Science and Therapeutic Potential”

16th – 17th January, 2013. The Royal Society, London, UK

Jointly organised by PPI-Net, RSC-BMCS, MGMS, SCI

http://ppi-net.org/events/

News: PROXO publication

20 October 2012

Rui Guo, Shaul Mukamel, David Klug. Geometry determination of complexes in a molecular liquid mixture using electron-vibration-vibration two-dimensional infrared spectroscopy with a vibrational transition density cube method. Phys Chem Chem Phys. 2012 Oct 28;14(40):14023-33. http://pubs.rsc.org/en/content/articlelanding/2012/cp/c2cp42593a

Event: BioTechniques Virtual Symposium 24 October 2012

18 October 2012

‘The Cell Landscape: From Genotypes to Phenotypes – the 2012 Virtual Symposium’

Symposium Overview
In recent years, new methods and techniques aimed at deciphering the inner workings of cells have provided unique insights, and changed the way we think about, as well as approach, modern cell biology research. With these advances in mind, the 2012 BioTechniques Virtual Symposium will focus directly on the latest approaches being used to characterize cell function and phenotype. From the genetic mechanisms controlling basic cellular processes to understanding how protein production and interactions shape cell structure and function – this one-day symposium will highlight techniques for teasing apart the genetic, proteomic, and metabolite landscapes of the cell.

Registration is free. Further information can be found at http://www.biotechniques.com/symposium/

News: PROXO member invited to present at SFRR-Europe Summer School

30 September 2012

Corinne M Spickett – Biochemistry of Redox Reactions. SFRR-Europe Summer School 2012 on Dietary factors and redox signalling, Spetses, Greece, 24-30th Sept 2012. http://www.sfrr-summer-school.org/

News: Adrenalin-fuelled Creativity

20 September 2012

Imperial College News

Dr Duncan Casey reports on the 2-day Creativity Training course.

“The course was held at Cumberland Lodge – a fantastic old venue in the shadow of Windsor Castle – and funded through a grant awarded to Dr Laura Barter (Chemistry) by the Postdoc Development Centre and the Institute of Chemical Biology at Imperial. It developed from the ‘sandpit’ events that the Engineering and Physical Sciences Research Council(EPSRC) has established, in which you’re thrown into a five-day, £10 million competition with around 30 total strangers to put together a proposal to work on a strategic, multidisciplinary target. It’s certainly an intense, high- adrenaline way to plan your next project.

The best, but also the hardest, thing about the idea is that you’re working with people with wildly different skill-sets and experience. I helped develop a synthetic biology proposal with a plastic electronics designer and an economist, amongst others. Fortunately, the event came equipped with a group of facilitators and trainers from EPSRC and the Biotechnology and Biological Sciences Research Council, and talks included one from an ex-editor at Nature, who spent a day helping cram some showmanship and theatricality into dry scientific presentations. It was great to hear that EPSRC would like postdocs to start appearing at the full sandpit events, and I’m very keen to get stuck into one.”

News: Third PROXO Project Meeting at Aston

15 September 2012

The third PROXO Project Meeting was held on 14 September 2012 at Aston University. The meeting was attended by staff and students from the Proxomics Project. After the welcome and introduction of new members of the Group (Dr Alexandra Alves Duarte, PDRA at Imperial and Drs Julien Reboud and Rab Wilson, PDRAs at Glasgow), the PDRAs presented scientific updates on their work for the project. Imperial and Glasgow students and PDRAs also had a tour of Aston laboratory facilities.

News: PROXO publication

12 September 2012

Julien Reboud, Yannyk Bourquin, Rab Wilson, Gurman S. Pall, Meesbah Jiwaji, Andrew R. Pitt, Anne Graham, Andrew P. Waters, and Jonathan M. Cooper. Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies PNAS 2012 ; published ahead of print September 4, 2012, doi:10.1073/PNAS 2012 109 (38) 15162-15167. http://www.pnas.org/content/early/2012/08/27/1206055109.abstract

In this paper, we demonstrate that phononic lattices microfabricated on a disposable device enable a tool-box of fluidic functions, by shaping surface acoustic waves (SAW). By controlling the geometry of the phononic structure, we show that we can combine different functions on a single platform, using the excitation frequency as a handle to switch between the different actuations. Here we focused on the detection of infectious disease in a drop of blood, using mouse malaria as a model, to demonstrate a complete nucleic acid-based test, including cell lysis, mixing, and thermal cycling on a SAW platform. This will be used to elucidate genetic cues in oxidative damage.

News: Proxo publication

20 August 2012

Lanigan PMP, Munro I, Grace EJ, Casey DR, Phillips J, Klug DR, Ces O, Neil MAA. Dynamical hologram generation for high speed optical trapping of smart droplet microtools. Biomedical Optics Express 2012, 3, 1609-1619.

http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-3-7-1609

News: PROXO member invited to present at 6th International Conference on Coherent Multidimensional Spectroscopy

20 July 2012

David Klug – Progress in Deconvolving Structural Elements In a Small Protein Using EVV 2DIR. 6th International Conference on Coherent Multidimensional Spectroscopy, Berlin 16-18 July 2012. http://www.mbi-berlin.de/cmds2012/index.html (Invited Talk)

News: PROXO member invited to present at Oxygen Club of California World Congress 2012 on Oxidants and antioxidants in Biology: Cell Signaling and Nutrient – Gene Interactions

23 June 2012

Corinne M Spickett – Lipid oxidation products: signalling and biomarkers for inflammatory disease. Oxygen Club of California World Congress 2012 on Oxidants and antioxidants in Biology: Cell Signaling and Nutrient – Gene Interactions, 20-23 June 2012, Alba, Italy.

News: Creativity Workshop for PDRAs Interested in Multidisciplinary Research

8 June 2012

PDRAs from the Proxomics Project took part in a 2-day residential Creativity Workshop on 6-7 June 2012. The workshop was designed to simulate “sandpit” events, a mechanism chosen by RCUK to bring together 20-30 scientists from a range of backgrounds for a residential creativity workshop, where participants generate radical solutions to address research challenges. The workshop featured training from the EPSRC and one of the leading research facilitation companies in the UK.

News: PROXO member invited to present at Stress in Health and Disease IV Spanish and Portuguese meeting on Free Radicals

7 June 2012

Corinne M Spickett – Harnessing mass spectrometry to investigate oxidative modifications of proteins. Stress in Health and Disease IV Spanish and Portuguese meeting on Free Radicals, Valencia 5-7th June 2012.

News: PROXO member invited to present at Proteomics Workshop 2012

6 June 2012

Corinne M Spickett – Protein Oxidative Modifications:  importance in pathophysiology and measurement. Proteomics Workshop 2012 Aveiro, Portugal, 4-6 June 2012.

News: PhD Studentships

11 May 2012

PhD Studentships are available at the Doctoral Training Centre in Cell & Proteomic Technologies, University of Glasgow.
www.gla.ac.uk/researchinstitutes/biology/infoforprospectivestudents/dtcinproteomictechnologies/

 

The advert can be viewed at:
www.findaphd.com/search/ProjectDetails.aspx?PJID=34316&LID=545

News: The second PROXO Project Meeting at Glasgow

30 April 2012

The second PROXO Project Meeting was held on 30 April 2012 at Glasgow. The meeting was attended by all members of the Proxomics Project. After introductions of new members of the Group (Dr Julia Davies, PDRA at Imperial, and Dr Craig Auchivole, PDRA at Glasgow), the Group carried out a planning exercise to formulate their workplan for the upcoming months to achieve the set objectives. Imperial and Aston students and PDRAs also had a tour of the Glasgow laboratory facilities.

The next project meeting will be at Aston University at Birmingham in September.

News: PROXO publication

20 April 2012

Julien Reboud,  Rab Wilson,  Yi Zhang,  Mohd H. Ismail,  Yannyk Bourquin and Jonathan M. Cooper. Nebulisation on a disposable array structured with phononic lattices, Lab Chip, 2012, 12, 1268-1273. http://pubs.rsc.org/en/content/articlelanding/2012/lc/c2lc20705b

We previously showed that surface acoustic waves (SAWs) carry a mechanical energy that can be used to nebulise biological samples off a surface, into the vapour phase, to be analysed by mass spectrometry in a soft manner, not requiring high voltages, a matrix or high laser powers.
In this paper, we demonstrate the use of a phononic lattice to shape acoustic fields on a disposable microfluidic chip to enable the nebulisation of samples in a sequential manner in an array format, using the excitation frequency to select the location within the array. The phononic design also enables to focus the energy, thus limiting the input power required and accelerating the process. This opens up applications of soft nebulisation in high-throughput areas such as proteomics.

News: The first PROXO Project Meeting at Imperial

25 January 2012

The first PROXO Project Meeting was held on 19 and 20 January 2012 at Imperial. The meeting was attended by all staff and students involved in the Proxomics project. After the welcome and introduction by the Chair of the PROXO Group, Professor David Klug, each project work stream leader presented a short talk to introduce the scientific and technological background and the objectives involved. Glasgow and Aston students and PDRAs were also shown round the Imperial laboratory facilities.

The next project meeting will be at Glasgow in April.

News: PROXO member invited to present at SPIE Photonics West, San Francisco

23 January 2012

Jon Cooper – Developing Diagnostics for the Developing World, SPIE Photonics West, San Francisco, USA, 23rd January 2012. http://spie.org/x85421.xml

News: Proxomics features headline of Glasgow University News

17 January 2012

The University of Glasgow released a headline news article featuring the Proxomics Project on the front page of the University website on Monday 16 January 2012.

http://www.gla.ac.uk/news/archiveofnews/2012/january/headline_220434_en.html

News: PROXO member invited to present at Novartis external seminar program

6 January 2012

Keith Willison – Microfluidic-based single molecule counting approaches to diagnostics, Novartis external seminar program, Novartis, Basel, 6th of January 2012.

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News: Job Opportunities at Imperial

20 December 2011

Current vacancies:

Research Associate in Coherent Laser Two Dimensional Infra-Red Spectroscopy of Proteins

Institute of Chemical Biology, Imperial College London

For more details, please visit the Jobs at Imperial website ((please select “Job Search” then enter the job title or vacancy reference number NS2011244RD into “Keywords” without any spaces).

Closing Date: 29 January 2012

News: Job Opportunities at Glasgow

15 December 2011

Current vacancies:

Research Assistant/Associate

College of Science & Engineering, School of Engineering, University of Glasgow

For more details, please visit the Jobs at Glasgow website

Closing Date: 18 January 2012

News: PROXO member invited to present at Analytical methods to study oxidative damage, antioxidants & drugs. Medical University of Bialystok

13 November 2011

Corinne M Spickett – Protein oxidation in stress and disease – finding the needle in the haystack. Analytical methods to study oxidative damage, antioxidants & drugs. Medical University of Bialystok, 10-13th November 2011.

News: Job opportunities

1 October 2011

Current vacancies:

Research Associate in Synthetic Chemical Biology

Imperial College London – Department of Chemistry, Faculty of Natural Sciences

Deadline: 24 October 2011

http://www.jobs.ac.uk/job/ADG713/research-associate-in-synthetic-chemical-biology/

News: Winner of the Proxomics Project Logo Competition

30 September 2011

Congratulations. David Patterson from the DTC in Cell and Proteomic Technologies at the University of Glasgow has won the Proxomics Project Logo Competition.

News: PhD studentships

20 September 2011

PhD studentships are available at

The Institute of Chemical Biology Doctoral Training Centre, Imperial College London

http://www3.imperial.ac.uk/chemicalbiology/doctoraltrainingcentre

and

The Doctoral Training Centre in Cell & Proteomic Technologies, University of Glasgow

http://www.gla.ac.uk/faculties/fbls/dtcinproteomictechnologies/

News: PROXO member invited to present at Society for Free Radical Research Europe 2011 Conference

10 September 2011

Corinne M Spickett – Protein oxidation in stress and disease – finding the needle in the haystack. Society for Free Radical Research Europe 2011 Conference Istanbul 7-10th September 2011.

News: Appointment of the Advisory Board

1 September 2011

A Board of leading scientists in both academia and industry have agreed to take up the advisory responsibility for the Proxomics project.

click on the link

The Proxomis Project Advisory Board members are:

• Dr Malcolm Weir, Heptares Therapeutics (Chair)

• Professor Julian Burke, Leica Microsystems

• Professor Philip Eaton, Kings’ College London

• Professor Walter Kolch, University College Dublin

• Professor Tony Ng, Kings’ College London

News: The Proxomics Project starts on 1 September 2011

1 September 2011

The Proxomics project was one of the three projects funded through the Cross Disciplinary Research Landscape Awards by the Engineering and Physical Sciences Research Council.  This is a £4.6 million multidisciplinary research collaboration between Imperial College London, University of Glasgow and Aston University to develop new technologies for understanding the causes of ageing and protein-oxidative damage. It builds upon the collaboration between the Institute of Chemical Biology Doctoral Training Centre at Imperial College London and the Doctoral Training Centre in Cell & Proteomic Technologies at the University of Glasgow. The project officially starts on 1 September 2011.

 

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